Biosynthesized Silver Nanoparticles Using Jojoba Oil as novel antibacterial agent for controlling Pectobacterium carotovorum in potato

Abstract

<title>Abstract</title> The current work describes an environmentally friendly way to create silver nanoparticles. Five bacterial isolates were isolated and tested for pathogenicity test, all of this isolates were able to produced typical symptoms of soft rot disease. Isolates Pcc2 caused the highest disease severity. According to 16sRNA it is identified as <italic>Pectobacterium carotovorum</italic> subsp. <italic>carotovorum.</italic> As a reducing and capping agent, jojoba oil was utilized in the production of silver nanoparticles (AgNPs). AgNPs may be identified by their UV-VIS, FTIR, and spectra. The transmission electron microscope was used to examine the shape and size of the harmless silver nanoparticles (TEM). The average particle size of the silver nanoparticles, according to the TEM data, was 25.6 ± 10.3 nm, and they had a spherical shape. The silver nanoparticles' surface had organic molecules bound to it, according to FTIR spectroscopy tests. Increasing the dosage of AgNPs had the greatest antibacterial effect against <italic>P. carotovorum</italic>, which produced soft rot on potatoes. From these results we can conclude that the use of AgNPs safety methods for controlling postharvest disease of potato.