Biomonitoring of Dietary Mycotoxin Exposure and Associated Impact on the Gut Microbiome in Nigerian Infants

Abstract

Mycotoxins are toxic chemicals that adversely affect human health. Here, we assessed the influence of mycotoxin exposure on the longitudinal development of early life intestinal microbiota of Nigerian neonates and infants (NIs). Human biomonitoring assays based on liquid chromatography tandem mass spectrometry were applied to quantify mycotoxins in breast milk (<i>n</i> = 68) consumed by the NIs, their stool (<i>n</i> = 82), and urine samples (<i>n</i> = 15), which were collected longitudinally from month 1-18 postdelivery. Microbial community composition was characterized by 16S rRNA gene amplicon sequencing of stool samples and was correlated to mycotoxin exposure patterns. Fumonisin B₁ (FB₁), FB₂, and alternariol monomethyl ether (AME) were frequently quantified in stool samples between months 6 and 18. Aflatoxin M₁ (AFM₁), AME, and citrinin were quantified in breast milk samples at low concentrations. AFM₁, FB₁, and ochratoxin A were quantified in urine samples at relatively high concentrations. <i>Klebsiella</i> and <i>Escherichia</i>/<i>Shigella</i> were dominant in very early life stool samples (month 1), whereas <i>Bifidobacterium</i> was dominant between months 3 and 6. The total mycotoxin levels in stool were significantly associated with NIs' gut microbiome composition (PERMANOVA, <i>p</i> < 0.05). However, no significant correlation was observed between specific microbiota and the detection of certain mycotoxins. Albeit a small cohort, this study demonstrates that mycotoxins may influence early life gut microbiome composition.