Biogenic synthesis, characterization, and antibacterial evaluation of silver nanoparticles synthesized from aqueous seed extract of Dolichos lablab

Abstract

Currently, green synthesis of AgNPs is an emerging technology over other conventional methods due to its low cost, energy efficiency, non-hazardousness, and environmental friendliness. The present study focuses on the green synthesis, characterization, and antibacterial activities of synthesized AgNPs using Dolichos lablab aqueous seed extract. Synthesis of AgNPs was done by using AEDLL AgNO3 solution at 70 ℃. The antibacterial activity was determined using the agar well diffusion method. Formation of AgNPs was confirmed by the appearance of the red brown color of the mixture and further confirmed by spectral analysis; meanwhile, parameters affecting the formation of AgNPs were also investigated. The optimum stirring time for AgNPs synthesis was found to be 45 min with 30 mL of 1mM AgNO3 solution and 15 mL AEDLL, which was favorable for the reduction of AgNPs. The UV-Vis spectrum of the optimized AgNPs shows a peak at 400 nm. FT-IR spectrum analysis indicates the presence of hydroxyl (-OH) functional groups in the synthesized AgNPs. The XRD pattern of AgNPs showed a face-centered cubic structure with an average particle size of 15.67 nm. The antibacterial activity revealed that Staphylococcus aureus was more susceptible to the treatment than Escherichia coli.