Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study)

Abstract

Purpose: This investigation aimed to assess the Biocompatibility and Marginal Adaptability of Bioceramic Putty material as root end filling material compared with Mineral Trioxide Aggregate (MTA).Methodology: For the marginal adaptability test, Thirty-six human extracted single rooted teeth were used. Samples were randomly distributed into 3 groups (n=12) based on the root end filling materials (Cerkamed Bio-MTA, Well-Root Putty, and Gutta percha). The tested materials were mixed and applied in accordance with the manufacturer's instructions. After complete setting, they were immersed into 1% Rhodamine B fluorescent dye for 24h then marginal adaptability was evaluated using confocal laser microscope measuring dye penetration in microns. For biocompatibility test, thirty-six discs with 2 mm thickness and 2 mm diameter were used. The discs were randomly distributed into 3 groups (n=12) consistent with the tested materials. Biocompatibility of the tested samples was measured against hFB (human fibroblast cells) for 24h and 48h using the MTT Cell Viability Assay.Results: For the marginal adaptability test, there were no statistically significant distinctions among Cerkamed Bio-MTA and Well-Root Putty. For biocompatibility test, Well-Root Putty samples showed significant cytotoxicity on both time intervals.Conclusion: Neither Cerkamed Bio-MTA and Well-Root putty showed 100% marginal adaptability but they were close to each other. Cerkamed Bio-MTA and Gutta percha did not affect cell viability whereas; Well-Root Putty showed cytotoxic effect on fibroblastic cells.