Biochemical Evaluation the Cardioprotective Effect of Ellagic acid and Resveratrol Against Methotrexate induced Cardiotoxicity in Male Rats

Abstract

The current work is aimed to explore the cardioprotective effect of Ellagic Acid (EA) and Resveratrol (RES) against Methotrexate (MTX) which causes cardiotoxicity. Rats were allocated into five groups as follows; Group I acted as a control, MTX group II, Group III received EA (30mg/kg b.w), Group IV received RES (25mg/kg b.w), Group V received EA plus RES as previous doses for 4 weeks. On 22nd day of EA and RES administration, all groups were injected with MTX (20 mg kg bw ip).4wks later, serum and cardiac tissues are collected for cardiac biomarkers measurement, cardiac histopathological picture and immunoexpressing of cardiac IL-6 and IL-1β. MTX-induced cardiotoxicity as confirmed through causing histopathological abnormalities of cardiac tissue, decreases the antioxidant defense system’s effectiveness in addition to significant elevation in cardiac biomarkers Treponin I, CKMB and LDH beside cardiac inflammatory cytokines IL-6 , IL-1β and CRP .MTX significantly increased serum AST, ALT activities, Creatinine and Urea levels. On the contrary, oral supplementation of EA or/and RES attenuated alterations in the above-mentioned biomarkers and cardiac histopathological changes. The present results summarized that EA and RES treatment showed cardioprotective effects against MTX toxicity. Combination between EA and RES gives best result as both increase total antioxidant capacity which protects cardiac tissue from MTX toxicity.