article · International Journal of Molecular Biology Open Access
Stem bark from Spondias mombin was air-dried, chopped, and subjected to exhaustive cold extraction with ethyl acetate. The resulting crude extract underwent solvent partitioning, and the dichloromethane fraction was separated using column chromatography with silica gel and Sephadex LH media to yield purified fractions. Analysis by gas chromatography mass spectrometry identified several distinct chemical compounds, including aspidofractinine methanol, phthalic acid ethylhexyl tetradecyl ester, phthalic acid dipropylpentyl ester, a substituted fluorine derivative, and terephthalic acid dodecyl ethylhexyl ester. Biological evaluations demonstrated that these isolated constituents possess both individual and synergistic antimicrobial efficacy against the Gram-negative bacterium Escherichia coli, the Gram-positive bacterium Bacillus subtilis, and the fungal pathogen Aspergillus flavus.
Identifying natural antimicrobial compounds from plant sources provides candidates for combating bacterial and fungal pathogens. Understanding how these isolated substances function individually and in combination helps inform the search for alternative antimicrobial agents, particularly as resistance to conventional treatments continues to challenge public health, medicine, and agricultural pest management.
The research demonstrates early-stage, laboratory-based discovery of bioactive plant compounds with antibacterial and antifungal actions. Potential applications could include active ingredients for pharmaceutical treatments or natural preservatives targeting specific microbial strains. However, the findings are at an early experimental level, and substantial validation, toxicological assessment, and formulation development would be necessary before any commercial pathway can be pursued.
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The purpose of this research work is to isolate purify chemical identification antimicrobial and Synegistic efficacy of extracted essential oils from ethyl acetate extract of Spondias mombin The ethyl acetate stem bark extract of Spondias mombin were air dried chopped into smaller pieces and cold extracted exhaustively with ethylacetate The crude extract was partitioned using various solvents and the dichloromethane fraction was concentrated and fractionated using column chromatography packed with Si gel and Sephadex LH and eluted with appropriate solvent systems accordingly In order to obtain pure extracts partially purified fractions were further purified The structures of the isolated compounds were determined by using data obtained from GC MS spectrum The compound isolated includeAspidofractinine methanol Phthalic acid ethylhexyl tetradecyl ester Phthalic acid di propylpentyl ester rsquo Dimethylpropanoilhydrazono dichloro bis diethylamino ethoxy fluorine and Tere phthalic acid dodecyl ethylhexyl ester These compounds have individual and synergistic activity against Gram negative E coli Gram positive Bacillus subtilis and Aspergillusflavus at and g mL
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DOI: 10.15406/ijmboa.2019.04.00110
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