Assessment of the salt tolerance of wheat genotypes during the germination stage based on germination ability parameters and associated SSR markers

Abstract

Although the germination stage accounts for a very short period of a plant’s life cycle, it involves numerous mechanisms and multistage processes that potentially differ among genotypes under salt stress. Therefore, we hypothesized that the parameters controlling the water uptake pattern and α-amylase activity during the seed germination process could be helpful for assessment the salt tolerance of wheat genotypes at the early growth stage. Genotypic differences in the germination ability parameters and α-amylase activity were assessed for seven wheat cultivars under normal and salt stress conditions at the molecular marker level using 30 simple sequence repeat (SSR) markers linked to salt tolerance. Results found that genotypic variations in the water uptake pattern were appeared at the late stage of phase II under both salinity levels and more obviously during phase III. Genotypic variations were observed in the germination time (GT), which was delayed by increasing salinity levels. The α-amylase activity and GT were positively and negatively correlated, respectively, with each time of water uptake rate. Significant correlation (r = 0.49, P = 0.026) was observed between similarity coefficients of germination ability parameters and SSR data based on the Mantel test. Among the 24 SSR markers, which showed polymorphism, Cdf 9, Cdf 46, Cdf 49, Wmc 503, and Gwm 312 were associated with almost germination ability parameters (R2 ranged from 0.43 to 0.95). Therefore, based on the molecular marker-phenotypic trait association at germination stage, assessment of salt tolerance in many wheat genotypes in a relatively short time could be conceivable.