Assessment of Nephroprotective and Antidiabetic Effects of Epipremnum aureum from Algeria in Albinos Rats

Abstract

Epipremnum is a remarkable medicinal herb that has been utilised in various traditional medical systems to cure human illnesses and afflictions. Numerous biochemical substances, including alkaloids, flavonoids, glycosides, terpenoids, and saponins, are said to be present. The objective of this work is to evaluate in vitro the antihyperglycaemic activity of the methanolic of Epipremnum aureum (EAMeOH) by the inhibition of two enzymes α-amylase and α-glucosidase and to evaluate in vivo the anti-nephrotoxic activity against by gentamicin induced nephrotoxicity. Different concentrations of extract were incubated with enzyme substrate solution and enzyme activity was measured. In addition, acarbose was used as a standard inhibitor. The extract was able to inhibit α-amylase and α-glucosidase with a percentage of 28.45 and 57.45% respectively with an IC50 of 222.37± 0.12 of α-glucosidase. For anti-nephrotoxic activity, five groups of 6 rats were treated respectively with CMC of sodium, EAMeOH (200 mg/Kg b.w), these treatments were administered orally 1h before the administration of gentamicin except the 1st group did not receive that CMC of sodium). The results showed that the administration of gentamicin decreased significantly (P≤0.05) the urine volume and the weight of the kidneys in control group, but the oral administration of the extract (200 mg/Kg b.w.) significantly increased (P≤0.05) the urine volume and the weight of the kidneys compared to the control. Furthermore, the treatment by EAMeOH extract for 7 days at a daily dose of 200 mg / kg b.w; causes the decrease of serum urea with 0.25 g /L with significant manner (P≤0.05) and creatinine with 7.45 mg /L compared to control group. Histopathological reports showed reduction in the damage of kidneys when treated with the extract. These results suggest that the Methanolic extract of E. aureum leaves may be useful in reducing the gentamicin induced nephrotoxicity.