Antioxidative Effect of Addiing Taurine and Leptin to Tris-Extenders on Freezing And Fertilizing Abilities of Buffalo-Semen

Abstract

Current study aimed to examine possible acts of adding taurine or leptin to extender on frezability and fertiablity of frozen buffalo-semen. Five sexually mature buffalo male were used for semen collection (twice/week for 35 days) using artificial vagina. Mass motility of all collected ejaculates was ≥70%. The collected semen was pooled, allotted into 7 replicates, then extended in Tris-egg yolk without (control), with taurine and leptin (20 and 40 ng/ml for each), or two combination of taurine (10 and 20 ng/ml) and leptin (20 and 20 ng/ml). Semen was frozen in straws for 2 wk and thawed at 370C/30 seconds. In diluted, equilibrated and thawed semen, the live, individual motility and abnormal sperm percentages were determined, while the hypo-osmotic swelling test (curled tail) was examine in semen after thawing. In the thawed seminal plasma, enzyme activity was assayed. Fertilizing ability of each semen treatment was determined. Results showed that adding 20 ng leptin/ml or 40 ng taurine/ml enhanced (P<0.05) all spermatozoa parameters after dilution, equilibration and thawing versus control and other treatments. Activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were reduced (P<0.05) with all additives compared with control semen. Pregnancy rate for buffalo cows which inseminated with extended semen contain 20 ng leptin /ml, 40 ng taurine /ml, and control was 90, 80 and 70% (P<0.05). Thia study concluded that, addition of 20 ng leptin/ml or 40 ng taurine/ml in the extender of Tris for buffalo frozen-semen had positive impact on sperm function during cryopreservation and increased fertility of buffalo-bull spermatozoa.