Antibacterial cross-streak activity by actinomycetes: ActinoData project

Abstract

Photographs taken of cross-streak agar plates with an antibiotic-producing actinomycete strain streaked in the middle of the plate, incubated for 7 days at 30 degrees Celsius (optimal growth temperature of actinomycetes), and the bacterial test strains (pathogens) streaked from the outer edge of the agar plate up to the actinomycete growth in the middle. Plates are incubated at 37 degrees Celsius (optimal growth temperature of the pathogenic strains) for 24hrs and inspected for activity. Gaps between the bacterial streak and the central growth streak serve as an indicator that the actinomycete produced a bioactive compound that inhibited the growth of the bacterium. The test bacterial strains (pathogens) streaked on the plate are in the following order:On the left, top to bottom:<i>Acinetobacter baumannii</i> ATCC 19606; <i>Klebsiella quasipneumoniae</i> ATCC 700603; <i>Staphylococcus aureus</i> ATCC 33591 (MRSA); <i>Enterococcus faecalis</i> ATCC 29212; and <i>Enterococcus faecalis</i> ATCC 51299 (VanB)On the right, top to bottom:<i>Acinetobacter baumannii</i> ATCC 1605; <i>Enterobacter cloacae</i> ATCC 1143; <i>Escherichia coli</i> ATCC 25922; <i>Pseudomonas aeruginosa</i> ATCC 27853; and <i>Staphylococcus aureus</i> ATCC 29213<br><br>Images are labelled as follow:actinomycete strain name_media type_Bacteria_agar<br>The actinomycete strain name will vary, while the media type could be M19 (g/L: 20 mannitol, 10 casamino acids, 20 peptone, 20 bacteriological agar, pH7.0) or ISP2 (g/L: 10 malt extract, 4 yeast extract, 4 glucose, 20 bacteriological agar, pH7.0). 'Bacteria' denotes that the analysis was performed on bacterial test strains, while 'agar' refers the the side of the plate visualized in the photograph.