article · Heliyon
This research assesses the chemical composition, antibacterial effects, and antioxidant activity of Verbascum sinaiticum leaf extract alongside silver nanoparticles synthesised from it. Chemical profiling identified more than 70 compounds in the plant extract, including flavonoids, fatty acids, phenolic compounds, and glycosides. The synthesised silver nanoparticles were spherical, measured between 2 and 40 nanometres, and exhibited peak absorbance at 408 nanometres. In laboratory tests against both Gram-positive and Gram-negative bacteria, both the raw extract and the nanoparticles displayed stronger inhibitory and bactericidal effects against Gram-positive strains. The silver nanoparticles demonstrated superior antibacterial performance compared to the extract alone. Conversely, antioxidant testing revealed that the unformulated leaf extract provided stronger free-radical scavenging capacity than the synthesised nanoparticles.
Bacterial resistance and oxidative stress drive demand for new bioactive formulations. Demonstrating that Verbascum sinaiticum can both supply therapeutic compounds and facilitate the synthesis of silver nanoparticles provides scientific evidence for using this traditional plant in developing new antimicrobial and antioxidant materials.
The findings point towards potential applications in topical antimicrobials, surface sanitisation, or natural health products. Pharmaceutical or biotechnology product developers targeting bacterial control could consider these materials for formulation pipelines. However, because the study is limited to in vitro laboratory testing, the technology is at an early research stage and requires extensive safety, stability, and in vivo efficacy testing before commercial use.
AI-generated from the published abstract. Always read the original work before citing.
The potential applications of plant extract and nanoparticles in antibacterial and antioxidant studies have garnered significant interest in recent times. Despite being utilized in Ethiopian traditional medicine, <i>Verbascum sinaiticum</i> (qetetina) constituents and its usage in nanoparticle synthesis remain relatively unexplored. This study explores the potential of the plant extract and its nanoparticles for antibacterial and antioxidant applications, with a focus on the leaf extracts and its silver nanoparticles. The leaf extract was analyzed using LC-MS and GC-MS and found to contain over 70 compounds, including glycosides, phenolic compounds, flavonoids, and fatty acids. The synthesized nanoparticles had a maximum absorbance of 408 nm, with a size range of 2-40 nm and showed a spherical shape. Using the agar well diffusion method, the extract and nanoparticles were evaluated against Gram-positive (<i>Staphylococcus aureus</i> ATCC 2592, <i>Streptococcus agalactia</i> ATCC12386) and Gram-negative bacteria (Acinetobacter baumannii ATCC19606, Pseudomonas aeruginosa ATCC27853) bacterial strains. In terms of antibacterial effects, both the silver nanoparticles and leaf extract displayed a greater impact on gram-positive bacterial strains over gram-negative bacterial strains. Additionally, the tests for lowest inhibitory and bactericidal concentration indicated similar outcomes. Notably, the silver nanoparticles exhibited greater antibacterial activity compared to the leaf extract alone. The DPPH (2, 2-diphenylpicrylhydrazyl) assay was conducted to investigate antioxidant activity. The results showed that the plant extract had an IC<sub>50</sub> value of 143 μg/ml, while the synthesized nanoparticle had an IC<sub>50</sub> value of 216 μg/ml, indicating that the plant extract had greater antioxidant activity than the synthesized silver nanoparticles.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.1016/j.heliyon.2024.e24215
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
