preprint · Zenodo (CERN European Organization for Nuclear Research)
The effectiveness of many antibiotics commonly used worldwide, has reduced, due to antibiotic resistance. The aim of this study was to test the antibacterial activity of a novel polyfunctional “metalloglyco-protein/polypeptide-organochlorine” bioflocculant, produced from Pseudomonas aeruginosa strain F29, accession number OQ734844, recently isolated from porcine feces, from an animal farm; on some selected, laboratory-obtained identified bacteria, namely, antibiotic-sensitive Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus SO183; and antibiotic-resistant Klebsiella pneumoniae, Enterobacter cloacae and methicillin-resistant Staphylococcus aureus SO180. Serial dilution and pour plate methods were used to isolate bacteria from porcine feces. Biochemical tests performed, identified probable Pseudomonas species. Flocculating activity tests revealed the bioflocculant from isolate F29 (Pseudomonas aeruginosa strain F29), possessed the highest value of 69%. Therefore, it was used for the study. This bioflocculant contained sodium, potassium, magnesium, saturated nitro compounds, sulfones, polysulfides; magnesium oxide, metal-chloride bonds and phosphorus-chlorine bonds; carboxyl, amide/peptide, aromatic alcohol, alkene and halo functional groups; and possessed a clustered and scaly surface on SEM. Antibacterial activity tests performed, using the agar well-diffusion method, revealed the bioflocculant was effective against Staphylococcus aureus SO183 at concentrations of 0.090 g/L and 0.150 g/L, and against the selected, identified Pseudomonas aeruginosa strain, only at a concentration of 0.150 g/L. The disk diffusion test, used for each selected, identified bacterium, also served as negative and positive controls, and revealed that all these bacteria, were antibiotic-resistant. This is the first report of a bioflocculant from Pseudomonas aeruginosa inhibiting another Pseudomonas aeruginosa strain, and it may be an effective alternative antibacterial agent.
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DOI: 10.5281/zenodo.18773936
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