Anti-thromboinflammation of Triherbal Extract in Diabetic Rats

Abstract

Objective: To evaluate the effects of a blended aqueous extract of Moringa oleifera, Ocimum gratissimum, and Vernonia amygdalina on some markers of thromboinflammation in diabetic rats. Material and Methods: We administered 150 mg/kg body weight of the individual extracts and the blended extract (1:1:1) to diabetic induced rats for 28 days. Acute toxicity of the extracts were determined by the Lorke technique while the markers of thromboinflammation, involving platelet count, mean platelet volume, platelet distribution width, plateletcrit, platelet large cell ratio, absolute white blood cell, lymphocytes, neutrophils, monocytes, basophils and eosinophils, were determined as part of the complete blood count using an automated hematology analyzer. Results: The LD50 of both the individual and blended extracts were observed to be above 5,000 mg/kg body weight. There was a significant increase in the serum glucose and markers of thromboinflammation involving the total white blood cell count, neutrophil count, platelet count, plateletcrit, mean platelet volume, platelet distribution width and the platelet large cell ratio, but a decrease in the lymphocyte and eosinophil count for the diabetic non-extract treated rats. The diabetic rats treated with the blended extract showed a significant restoration of the parameters. Conclusion: This study revealed that the blended aqueous extracts of Moringa oleifera, Ocimum gratissimum, and Vernonia amygdalina are non-toxic and may have synergistic effects in limiting thromboinflammation in a diabetic state.