review · Critical Reviews in Analytical Chemistry
Quercetin is a dietary flavonoid found across various fruits, vegetables, and drinks, recognised for health benefits such as antioxidative, anti-inflammatory, anti-mutagenic, and anti-carcinogenic properties. Accurately measuring quercetin and its glycosides across diverse matrices, notably pharmaceutical formulations and biological specimens, requires effective sample preparation and analytical techniques. Different analytical strategies offer distinct operational trade-offs. Ultraviolet-visible spectrophotometry provides an inexpensive and straightforward approach, though it suffers from limited selectivity. In contrast, chromatographic techniques, including high-performance liquid chromatography and gas chromatography, deliver enhanced selectivity and sensitivity. Electrophoretic approaches yield high resolution, while electrochemical methods achieve notably low detection limits. Evaluating these diverse methodologies provides a clear framework for selecting and optimising analytical protocols to monitor and measure quercetin and its derivatives in real-world samples.
Quercetin is widely consumed and researched for its potential protective health properties against inflammation, oxidation, and cancer. However, verifying its presence and concentration in medications or biological fluids demands reliable measurement tools. Comparing the practical strengths and weaknesses of different analytical techniques helps laboratories choose the right testing method, supporting better quality control in health products and more dependable biological research.
The work guides the selection and development of testing protocols for pharmaceutical quality control laboratories and biological testing facilities. Analytical techniques such as chromatography and electrochemistry are already established in commercial and diagnostic practice, meaning the review addresses applied and tested methods rather than novel laboratory concepts. These insights assist commercial testing laboratories and drug developers in standardising quality assurance procedures for quercetin-based formulations and diagnostics.
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Flavonoids are plant-derived compounds that have several health benefits, including antioxidative, anti-inflammatory, anti-mutagenic, and anti-carcinogenic effects. Quercetin is a flavonoid that is widely present in various fruits, vegetables, and drinks. Accurate determination of quercetin in different samples is of great importance for its potential health benefits. This review, is an overview of sample preparation and determination methods for quercetin in diverse matrices. Previous research on sample preparation and determination methods for quercetin are summarized, highlighting the advantages and disadvantages of each method and providing insights into recent developments in quercetin sample treatment. Various analytical techniques are discussed including spectroscopic, chromatographic, electrophoretic, and electrochemical methods for the determination of quercetin and its derivatives in different samples. UV-Vis (Ultraviolet-visible) spectrophotometry is simple and inexpensive but lacks selectivity. Chromatographic techniques (HPLC, GC) offer selectivity and sensitivity, while electrophoretic and electrochemical methods provide high resolution and low detection limits, respectively. The aim of this review is to comprehensively explore the determination methods for quercetin and quercetin glycosides in diverse matrices, with emphasis on pharmaceutical and biological samples. The review also provides a theoretical basis for method development and application for the analysis of quercetin and quercetin glycosides in real samples.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.1080/10408347.2023.2269421
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