Additional file 10 of Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex

Abstract

Supplementary Material 10. Supplementary Figure 10: Knockdown of TEP1 has no impact on pyrethroid resistance in Tiassalé_Ag.sl. A. Bar graphs depicting transcript levels (y-axis) of TEP1 after dsRNA injection (grey bars), normalized to the GFP-injected control (black bars) 72- and 96-h post injection. Depicted values are the average of three biological replicates with three technical replicates each, ± SD. Statistical comparison between control and RNAi group were calculated with unpaired t-test: * p < 0.05 B. Bar chart showing comparison of quantified TEP1 (processed) levels between control and TEP1 dsRNA injected mosquitos, after normalization of respective signal to the α-tubulin loading control. Western blot of TEP1 proteins in control dsGFP (left) and dsTEP1 (right) injected mosquitos over a time course, below is the α-tubulin loading control. d3 and d5 refer to day 3- and 5-post-injection. C. Kaplan-Meier-plot comparing the survival of non-injected (black) versus dsTEP1-injected mosquitoes (red). The vertical line represents time of injection. 50 female mosquitos were used per group. D. Observed 24 h mortality of pyrethroid-resistant after 1 h exposure to a control tube or 0.05 % deltamethrin in a WHO-tube assay. Percentage mortality (y axis) for control exposed for each injection group (black), dsGFP (grey) and dsTEP1 (red). Statistical comparison between control and deltamethrin exposed group were calculated with unpaired t-test. Sample size is indicated below bars. All experiments done with Tiassalé_Ag.sl.