article · Chemical Communications

A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein–protein interactions

Clinton G. L. Veale
Richwell Mhlanga
Fernando Alberício
Beatriz G. de la Torre
Abir Chakraborty
Adrienne L. Edkins
David J. Clarke

20242 citationsOpen accessRhodes University

Abstract

Native mass spectrometric analysis of TPR2A and GrpE with unpurified peptides derived from limited proteolysis of their respective PPI partners (HSP90 C-terminus and DnaK) facilitated efficient, qualitative identification of interfacial epitopes involved in transient PPI formation. Application of this approach can assist in elucidating interfaces of currently uncharacterised transient PPIs.

Research topics

Mass Spectrometry Techniques and Applications
Advanced Proteomics Techniques and Applications
Enzyme Structure and Function

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DOI: 10.1039/d4cc01251h

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